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Monkey CD31/PECAM-1 ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP1-92720

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NBP1-92720

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (baboon) (10 uL)

Sensitivity

0.06 U/mL

Assay Range

0.48 - 30.0 U/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Monkey

Description

The monkey sPECAM-1 ELISA is an enzyme-linked immunosorbent assay for quantitative detection of soluble monkey platelet endothelial cell adhesion molecule-1.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 2.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Monkey CD31/PECAM-1 ELISA Kit (Colorimetric)

Recovery

The overall mean recovery was 110 %. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Monkey CD31/PECAM-1 ELISA Kit (Colorimetric)

ELISA: Monkey, CD31/PECAM-1 ELISA Kit (Colorimetric) [NBP1-92720] - Standard curve using Monkey CD31/PECAM-1 ELISA Kit (Colorimetric)

Kit Contents for Monkey CD31/PECAM-1 ELISA Kit (Colorimetric)

  • Adhesive Plate Covers
  • Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey sPECAM-1, and HRP-Conjugate (anti monkey sPECAM-1 monoclonal (murine) antibody), Sample Diluent lyophilized
  • Aluminium pouch(es) with a monkey sPECAM-1 Standard curve (coloured)
  • Sample Diluent (Use when an external predilution of the samples is needed)
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Monkey CD31/PECAM-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Monkey CD31/PECAM-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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