Aldosterone Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE016
Discontinued Product
KGE016 has been discontinued.
View all Aldosterone products.
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (34 µL), Serum (34 µL), EDTA Plasma (34 µL), Heparin Plasma (34 µL), Urine (10 µL)
Sensitivity
22.4 pg/mL
Assay Range
24.7-6000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Aldosterone Parameter Assay Kit
The Parameter Aldosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Aldosterone in cell culture supernates, serum, plasma, and urine.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures Aldosterone levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 278 | 878 | 2536 | 263 | 832 | 2419 |
| Standard Deviation | 9.49 | 17.3 | 34.4 | 31.7 | 83.0 | 230 |
| CV% | 3.4 | 2.0 | 1.4 | 12.1 | 10.0 | 9.5 |
Recovery for Aldosterone Parameter Assay Kit
The recovery of Aldosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 89-99 |
| EDTA Plasma (n=4) | 89 | 76-110 |
| Heparin Plasma (n=4) | 89 | 79-107 |
| Serum (n=4) | 88 | 78-108 |
| Urine (n=4) | 101 | 95-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Aldosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as directed in the Sample Preparation section.
Scientific Data Images for Aldosterone Parameter Assay Kit
Multi-species Aldosterone ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Aldosterone
Additional Aldosterone Products
Product Documents for Aldosterone Parameter Assay Kit
Product Specific Notices for Aldosterone Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.