StemXVivo Mouse Pluripotent Stem Cell Media Kit

Kit Summary

Supports the long term culture of mouse pluripotent stem cells under feeder-free conditions.

Why use StemXVivo^® Mouse Pluripotent Stem Cell Media to grow mouse pluripotent stem cells?

Maintenance and expansion of mouse embryonic or induced pluripotent stem cells in vitro is a time consuming and sensitive process that, if not optimized, can yield inconsistent growth and cells of varying quality.

Critical to the in vitro culturing of mouse pluripotent stem cells is the use of an optimized media that includes mouse Leukemia Inhibitory Factor (LIF). R&D Systems offers the Mouse StemXVivo^® Mouse Pluripotent Stem Cell Media Kit, which provides a pre-optimized base media and our in-house produced and Quality Control-tested Recombinant Mouse LIF. Click here to learn more about R&D Systems^® Recombinant Mouse LIF. By combining our optimized base media with a highly bioactive LIF, this kit provides a nutrient environment for mouse embryonic and induced pluripotent stem cells that is ideal for maintenance and expansion.

StemXVivo^® Mouse Pluripotent Stem Cell Media Kit:

• Includes premium quality media and growth factors
• Features R&D Systems^® Recombinant Mouse LIF
• Optimized for both mouse embryonic and induced pluripotent stem cells
• Maintains tri-lineage germ layer competency
• High lot-to-lot consistency

This kit contains the following reagents to maintain mouse pluripotent stem cells in culture:

• Mouse Pluripotent Stem Cell Base Media (250 mL)
• Recombinant Mouse LIF (1000X)

Stability and Storage

Store unopened reagents in the dark at -20 ^°C in a manual defrost freezer. Do not use past the expiration date.

Precaution

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.
• Results may vary due to variations among cells derived from different donors.

Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express Pluripotency Stem Cell Markers, SSEA-1, Oct-3/4, and Alkaline Phosphatase, and Lack SSEA-4. v6.5 mouse embryonic stem cells (Novus Biologicals, Catalog # NBP1-41162) were cultured in StemXVivo® Mouse Pluripotent Stem Cell Media. A. Expression of SSEA-1 and SSEA- 4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A). B. Expression of Oct-3/4 was detected using Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (R&D Systems, Catalog # MAB1759) followed by NorthernLights™ (NL)557-conjugated Goat Anti-Rat Secondary Antibody (R&D Systems, Catalog # NL013). C. Expression of Alkaline Phosphatase was detected using Goat Anti-Mouse Alkaline Phosphatase Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF2910) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001). The nuclei were counterstained with DAPI.

Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in StemXVivo® Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex® BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. A. Expression of the ectoderm marker, SOX1, was detected in day 10 cells using Goat Anti-SOX1 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF3369) followed by NorthernLights™ (NL)557-conjugated Donkey Anti-Goat Secondary Antibody. B. Expression of the endoderm marker, SOX17, was detected in 10 day cells using Goat Anti-SOX17 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF1924) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody. C. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin Monoclonal Antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.