What is GlutaminePlus, and how does it compare to L-Glutamine?

Name: DMEM/F-12, 15 mM HEPES, GlutaminePlus
Brand: R&D Systems
SKU: M23950

GlutaminePlus, a derivative of L-glutamine obtained by a chemical reaction of L-alanine with L-glutamine, is available in liquid form or as an ingredient in ready-to-use Atlanta Biologicals brand cell culture media (media with stable L-glutamine).GlutaminePlus is metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.L-glutamine is an essential amino acid and plays a major role for the growth and function of cells in culture. Although L-glutamine is stable in crystalline form, it has the tendency to degrade non-enzymatically and irreversibly in solution within a short time period. The rate of this L-glutamine breakdown is dependent on pH, temperature and the presence of various anions.One of the by-products, ammonia, may act as a toxin or growth inhibitor for the cultured cells. Because of its chemical instability and significance for cell growth, it is important that the delivery of L-glutamine be optimized to each specific cell culture application. A recommended way of achieving reliable delivery of L-glutamine to the cells in culture is the use of stable derivatives of L-glutamine in cell culture media.

DMEM/F-12, 15 mM HEPES, GlutaminePlus

R&D Systems, part of Bio-Techne | Catalog # M23950

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Product Datasheet / COA / SDS

This item is in process of being discontinued.

Key Product Details

Features:

Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) with 15 mM HEPES buffer and GlutaminePlus.

Discontinued Product

M23950 has been discontinued. View all Cell Culture Media products.

Technical Data
Citations / Reviews / FAQs

Summary for DMEM/F-12, 15 mM HEPES, GlutaminePlus

What is DMEM/F-12 Media?

Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12) is a 1:1 mixture of DMEM and F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s Nutrient Mixture F-12’s additional components, was found to support the growth of a wide range of mammalian cells. DMEM/F-12 requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.

Each lot of DMEM/F-12 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of DMEM/F-12 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. DMEM/F-12 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.

Product Specifications for DMEM/F-12, 15 mM HEPES, GlutaminePlus

Applications	Cell Culture
Formulation	Liquid
Purification	0.2 μm filtered
Sterility Testing	Pass

Preparation & Storage

Shipping Conditions	Ambient
Storage	2 - 8 °C, protected from light

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DMEM/F-12, 15 mM HEPES, GlutaminePlus

R&D Systems, part of Bio-Techne | Catalog # M23950

Key Product Details

Summary for DMEM/F-12, 15 mM HEPES, GlutaminePlus

What is DMEM/F-12 Media?

Product Specifications for DMEM/F-12, 15 mM HEPES, GlutaminePlus

Preparation & Storage

Customer Reviews for DMEM/F-12, 15 mM HEPES, GlutaminePlus

FAQs for DMEM/F-12, 15 mM HEPES, GlutaminePlus

Product Documents for DMEM/F-12, 15 mM HEPES, GlutaminePlus

Product Datasheets

COA

Certificate of Analysis

SDS

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