How should Cultrex Basement Membrane Extract (BME) be stored and handled?

For best practices for handling our BME products, please refer to our helpful guide at the following link: https://www.bio-techne.com/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins/cell-culture-matrix-for-your-research

What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

Cultrex RGF Basement Membrane Extract, Type 2 (Phenol Red)

Name: Cultrex RGF Basement Membrane Extract, Type 2 (Phenol Red)
Brand: R&D Systems
SKU: 3533-005-02P

R&D Systems, part of Bio-Techne | Catalog # 3533-005-02P

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Product Datasheet / COA / SDS

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

Discontinued Product

3533-005-02P has been discontinued. View all Basement Membrane Extracts products.

Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Applications

Bioactivity

Formulation, Preparation, and Storage

Formulation

12 - 18 mg/mL.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Product is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles.

Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate.

Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines.

Background: Basement Membrane Extracts

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan. BME can be used in a multiple applications, under a variety of cell culture conditions, for maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle and stem cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.

Recently we have developed two additional formulations of Cultrex® BME known as Cultrex® BME Type 2 and Cultrex® BME Type 3. Cultrex® BME Type 2 provides a proprietary formulation that is higher in tensile strength when compared to our original BME, while Cultrex® BME Type 3 is physiologically aligned with the in vivo solid tumors environment and is recommended for xenografts and other in vivo applications.

There are many applications for Cultrex® BME, which require different thicknesses and concentrations. In general, BME at a protein concentration ≥ 10 mg/ml is used for differentiation studies of primary cells. For applications such as endothelial cell formation of capillary-like structures (Tube Formation Assay), the differentiation of rat aorta tissue into capillary-like structures (Aortic Ring Assay), epithelial organoid formation, or tumor organoid formation, a thick gel is needed. Some applications, such as propagation of primary cells, require a thin layer coating and not a thick gel; therefore, the thin layer method should be used.

Additional Basement Membrane Extracts Products

Product Documents for Cultrex RGF Basement Membrane Extract, Type 2 (Phenol Red)

Product Datasheets

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COA

SDS

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Product Specific Notices for Cultrex RGF Basement Membrane Extract, Type 2 (Phenol Red)

For research use only

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