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CellXVivo Human M1 Macrophage Differentiation Kit

For the differentiation of human CD14+ monocytes into M1 macrophages
Optimized Base Media in CellXVivo™ Macrophage Differentiation Kits Results in More Efficient Differentiation of M1 and M2 Macrophages.
(4)
Phenotypic Analysis of Human M1 Macrophages. 
Differentiated Human M1 Macrophages Secrete IL-12. 
CellXVivo Human M1 Macrophage Differentiation Kit
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CDK012

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, M1 macrophages can be generated from human monocytes using the following procedure:

  • Isolate human CD14+ monocytes from a PBMC preparation
  • Culture CD14+ monocytes in M1 Macrophage Differentiation Media
  • Verify differentiation into M1 macrophages by flow cytometry
  • Verify differentiation into by ELISA detection of IL-12
 

 

Reagents Provided

Reagents Supplied in the CellXVivo™ Human M1 Macrophage Differentiation Kit (Catalog # CDK012):

  • Serum-Free Base Media
  • Recombinant Human GM-CSF
  • Reconstitution Buffer 2

 

Other Supplies Required

Reagents

  • MagCellect™ Human CD14+ Cell Isolation Kit (R&D Systems, Catalog # MAGH105, or equivalent).
  • Ficoll-Hypaque™
  • Penicillin (optional)
  • Streptomycin (optional)
  • Cell Dissociation Solution Non-enzymatic 1x (Sigma)
  • Lipopolysaccaride (LPS) (optional)

Equipment

  • Tissue culture flasks and/or plates
  • Inverted microscope
  • Hemocytometer
  • 37 °C and 5% CO2 humidified cell culture incubator
  • Centrifuge
  • Pipettes and pipette tips

 

Procedure Overview

Isolate PBMCs from human blood.

Isolate PBMCs from human blood

Enrich human CD14+ monocytes from PBMCs (e.g., using magnetic cell selection).

Isolate T Cells from PBMCs

Perform a cell count.

Perform a cell count

Verify M1 macrophage differentiation on day 6 by analyzing cell surface marker expression via flow cytometry.

M1 macrophages are ready for downstream application.

Verify Th2 cell differentiation

Citations

Currently there are no citations for CellXVivo Human M1 Macrophage Differentiation Kit.

FAQs

  • Has the CellXVivo Human M1 Macrophage differentiation kit (Catalog # CDK012) been tested with human monocytic cell lines?

    We have only tested the CellXVivo Human M1 Macrophage differentiation kit with primary CD14+ monocytes derived from PBMCs.

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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