Discontinued Product
5881 has been discontinued.
View all Buffers, Chelators and Reagents products.
Key Product Details
Description
General purpose buffer
Product Description
Bicine is a widely used general purpose buffer for biological research. Working pH range in aqueous solution: 7.6-9.0. Used in protein crystallization, ion exchange chromatography and SDS-PAGE.Product Specifications
Molecular Weight
163.17
Formula
C6H13NO4
Storage
Store at RT
Purity
≥99%
Chemical Name
N,N-Bis(2-hydroxyethyl)glycine
CAS Number
150-25-4
PubChem ID
8761
InChI Key
FSVCELGFZIQNCK-UHFFFAOYSA-N
SMILES
OC(CN(CCO)CCO)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 326.34 | 2000 |
Preparing Stock Solutions
for Bicine
The following data is based on the product molecular weight 163.17
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 20 mM | 0.31 mL | 1.53 mL | 3.06 mL |
| 100 mM | 0.06 mL | 0.31 mL | 0.61 mL |
| 200 mM | 0.03 mL | 0.15 mL | 0.31 mL |
| 1000 mM | 0.01 mL | 0.03 mL | 0.06 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for Bicine
For research use only
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