WT1 Antibody (CL13170) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44378
![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202512295671.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202512145340.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL13170
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P19544, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for WT1 Antibody (CL13170) - Azide and BSA Free
![WT1 Antibody (CL13170) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-2822025127370.jpg "Immunocytochemistry/ Immunofluorescence: WT1 Antibody [NBP3-44378]")
Immunocytochemistry/ Immunofluorescence: WT1 Antibody [NBP3-44378]
Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody) .![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202512332975.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]
Staining of human kidney shows strong nuclear positivity in cells in glomeruli.![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202512495854.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-28220251375669.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202513121874.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202513112883.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
![WT1 Antibody (CL13170) - Azide and BSA Free Western Blot: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202513381589.jpg "Western Blot: WT1 Antibody [NBP3-44378]")
![WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]](https://resources.bio-techne.com/images/products/nbp3-44378_mouse-wt1-mab-cl13170-azide-and-bsa-free-282202513341195.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]")
Applications for WT1 Antibody (CL13170) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WT1
Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.
Long Name
Wilms Tumor 1
Alternate Names
GUD, WAGR, WIT-2, WT33
Gene Symbol
WT1
Additional WT1 Products
Product Documents for WT1 Antibody (CL13170) - Azide and BSA Free
Product Specific Notices for WT1 Antibody (CL13170) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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