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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A6

Format

BSA Free

Concentration

1 mg/ml

Product Summary for VLDLR Antibody (6A6) - BSA Free

Immunogen

Synthetic peptide corresponding to the C-terminus of human VLDL Receptor [Swiss-Prot# P98155]

Localization

Membrane; Single-pass type I membrane protein. Membrane > clathrin-coated pit; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VLDLR Antibody (6A6) - BSA Free

Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.
Immunocytochemistry/Immunofluorescence: VLDLR Antibody (6A6) [NBP1-78162] - VLDL R Antibody (6A6) [NBP1-78162] - VLDL Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor in mouse kidney.

Applications for VLDLR Antibody (6A6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000
Application Notes
This VLDL Receptor (6A6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where various isoforms can be detected between 120 and 168 kDa (see reference Jokinen et al, 1994).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-78162 in the following applications:

Published Applications

Read 7 publications using NBP1-78162 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VLDLR

VLDL (very low density lipoprotein) cholesterol is one of the three major types of cholesterol found in blood. VLDL receptor plays an essential role in triglyceride metabolism. It binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.

Long Name

Very Low Density Lipoprotein Receptor

Alternate Names

CHRMQ1, VLDL R, VLDLRCH

Entrez Gene IDs

7436 (Human); 22359 (Mouse); 25696 (Rat)

Gene Symbol

VLDLR

UniProt

Additional VLDLR Products

Product Documents for VLDLR Antibody (6A6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VLDLR Antibody (6A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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