VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16893

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VEGFR2/KDR/Flk-1 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y1175 of human VEGFR2/KDR/Flk-1 (NP_002244.1). KDYIV

Modification

p Tyr1175

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] AntibodyAzide and BSA Free [NBP3-16893]

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] AntibodyAzide and BSA Free [NBP3-16893]

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody [NBP3-16893] - Western blot analysis of extracts of NIH/3T3 cells, using VEGFR2/KDR/Flk-1 antibody (NBP3-16893) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free [NBP3-16893] -

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free [NBP3-16893] - Western blot analysis of lysates from A-431 cells, using VEGFR2/KDR/Flk-1 Rabbit pAb at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free [VEGFR2/KDR/Flk-1] -

Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free [VEGFR2/KDR/Flk-1] - Western blot analysis of lysates from A-431 cells, using VEGFR2/KDR/Flk-1 Rabbit pAb at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Applications for VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VEGFR2/KDR/Flk-1

Vascular endothelial growth factor receptor 2 (VEGFR2) is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other common growth factor receptors, VEGF receptor 2 dimerises upon ligand binding and is autophosphorylated at multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or can serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Defects in the VEGFR2 gene are associated with susceptibility to benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium called hemangioma capillary infantile. HCI is the most common tumor found in infants, found in up to 10% of all births.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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