Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HWA4C4

Format

BSA Free

Concentration

0.5 mg/ml

View all Ub-K63 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is Ub-K63.

Specificity

HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Ub-K63 Antibody (HWA4C4) - BSA Free

Western Blot: Ub-K63 Antibody (HWA4C4)BSA Free [NBP2-00199]

Western Blot: Ub-K63 Antibody (HWA4C4)BSA Free [NBP2-00199]

Western Blot: Ub-K63 Antibody (HWA4C4) [NBP2-00199] - In vitro linked and purified polyubiquitin chains were analyzed by SDS-PAGE followed by immunoblotting with Anti-Human Ub-K63 Purified. The antibody detects lysine-63-linked chains (lane 1), but does not detect lysine-48-linked chains (lane 2).

Applications for Ub-K63 Antibody (HWA4C4) - BSA Free

Application
Recommended Usage

Western Blot

< = 2 ug/ml
Application Notes
This HWA4C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains. This can be used at less than or equal to 2ug/ml . It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ub-K63

Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.

Additional Ub-K63 Products

Product Documents for Ub-K63 Antibody (HWA4C4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ub-K63 Antibody (HWA4C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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