Tryptophan hydroxylase 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.

Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot LUT. Image from verified customer review.

Immunoprecipitation: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555] -

The 5-HT and TPH2 in the raphe nuclei of the brain. The 5-HT- and TPH2-positive double stained cells in the dorsal raphe nuclei (A). Representative photomicrographs were taken at magnifications of 100×. Quantification of 5-HT and TPH2 (B). Data are expressed as the means ± SD (n = 3). *, p < 0.05; **, p < 0.01 compared with the vehicle group. 5-HT; 5-hydroxytryptamine, TPH2; tryptophan hydroxylase, DAPI; 4′,6-diamidine-2-phenylindole dihydrochloride.

Western Blot: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555] -

Enhanced serotonergic phenotypes of differentiated HT22 cells. (A) Immunofluorescence staining of SERT (green), 5-HT1aR (orange) and TPH2 (magenta) in hippocampal primary culture. DAPI staining (blue) indicates the nucleus. Scale bar = 50 μm. (B,C) SERT (green), 5-HT1aR (orange) and TPH2 (magenta) immunofluorescence staining (B) and western blotting (C) of HT22 cells grown in normal growth medium and differentiation medium for 3 d. DAPI staining (blue) indicates the nucleus. Scale bar = 25 μm. GAPDH was used as loading control. Data are representative of at least three independent experiments and are quantified using densitometric analysis. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36703746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555] -

Pet-1 enhances the serotonergic phenotype in differentiated HT22 cells. (A) Immunofluorescence staining of Pet-1 (red) in hippocampal primary culture. DAPI staining (blue) indicates the nucleus. Scale bar = 50 μm. (B,C) Pet-1 (red) immunofluorescence staining (B) and western blotting (C) of HT22 cells grown in normal growth medium and differentiation medium for 3 d. DAPI staining (blue) indicates the nucleus. Scale bar = 25 μm. GAPDH is used as loading control. (D,E) Control siRNA and Pet-1 siRNA were transiently transfected in HT22 cells, changed with normal growth medium and differentiation medium for 3 d. Western blotting for analyzing Pet-1 (D) and SERT, 5-HT1aR, and TPH2 (E) expression. GAPDH, vinculin, and beta-actin were used as loading controls. Data are representative of at least three independent experiments and are quantified using densitometric analysis. Statistical significance: *p < .05, and ***p < .001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36703746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555] -

Functional loss of 5-HT1Areceptor on the corticosterone and thermoregulatory responses. After fluoxetine injection for 4 weeks, the protein expression of TPH2 and c-Fos following 15 min of 8-OH-DPAT challenge was determined by western blot analysis in RN (A and B). Plasma corticosterone levels (C) or rectal temperature measurement (D) following 0, 15, 30, or 45 min of challenge with 8-OH-DPAT were analyzed. In addition, the plasma corticosterone levels were determined both pre- and post-exercise (E). The data are expressed as the mean +/- SD (n = 4 or 8/group). *p < 0.05 and **p < 0.01 compared to the saline group, #p < 0.05 and ##p < 0.01 compared to the saline-injected group without 8-OH-DPAT challenge or exercise. Serum cortisol levels of patients with ME/CFS were determined by EIA (F). The data are expressed as the mean +/- SD (n = 10 and 17, respectively). *p < 0.05 compared to the healthy controls Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38191373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555] -

The 5-HT and TPH2 in the raphe nuclei of the brain. The 5-HT- and TPH2-positive double stained cells in the dorsal raphe nuclei (A). Representative photomicrographs were taken at magnifications of 100×. Quantification of 5-HT and TPH2 (B). Data are expressed as the means +/- SD (n = 3). *, p < 0.05; **, p < 0.01 compared with the vehicle group. 5-HT; 5-hydroxytryptamine, TPH2; tryptophan hydroxylase, DAPI; 4′,6-diamidine-2-phenylindole dihydrochloride. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31906307), licensed under a CC-BY license. Not internally tested by Novus Biologicals.