TROP-2 Antibody (001) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89492
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492]](https://resources.bio-techne.com/images/products/TROP-2-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-89492-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Primate
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 001
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human TROP-2 (Accession#: NP_002344.2; Met 1-Thr 274).
Specificity
Has cross-reactivity in ELISA with: Human E-cadHuman CD146Human CD171Human BCAMHuman ICAM1No cross-reactivity in ELISA with: Human VCAM1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TROP-2 Antibody (001) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492]
Immunocytochemistry/Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] - Staining of human TROP2 in MCF-7 cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of MCF-7 cells.![Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492]](https://resources.bio-techne.com/images/products/TROP-2-Antibody-001-Flow-Cytometry-NBP2-89492-img0002.jpg "Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492]")
Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492]
Flow Cytometry: TROP-2 Antibody (001) [NBP2-89492] - Analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] -
Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody (001) [NBP2-89492] - Immunofluorescence staining of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89492 (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cell membrane.Applications for TROP-2 Antibody (001) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TROP-2
Long Name
Tumor-associated Calcium Signal Transducer 2
Alternate Names
GA733-1, gp50, T16, TACSTD2, TROP2
Gene Symbol
TACSTD2
Additional TROP-2 Products
Product Documents for TROP-2 Antibody (001) - Azide and BSA Free
Product Specific Notices for TROP-2 Antibody (001) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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