TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81074

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP2-81074AF350

Alexa Fluor 405

NBP2-81074AF405

Alexa Fluor 488

NBP2-81074AF488

Alexa Fluor 532

NBP2-81074AF532

Alexa Fluor 594

NBP2-81074AF594

Alexa Fluor 647

NBP2-81074AF647

Alexa Fluor 700

NBP2-81074AF700

Alexa Fluor 750

NBP2-81074AF750

Biotin

NBP2-81074B

CoraFluor 1

NBP2-81074CL1

DyLight 350

NBP2-81074UV

DyLight 405

NBP2-81074V

DyLight 488

NBP2-81074G

DyLight 550

NBP2-81074R

DyLight 594

NBP2-81074DL594

DyLight 650

NBP2-81074C

DyLight 680

NBP2-81074FR

DyLight 755

NBP2-81074IR

FITC

NBP2-81074F

HRP

NBP2-81074H

Janelia Fluor 525

NBP2-81074JF525

Janelia Fluor 549

NBP2-81074JF549

Janelia Fluor 646

NBP2-81074JF646

Janelia Fluor 669

NBP2-81074JF669

mFluor Violet 450 SE

NBP2-81074MFV450

mFluor Violet 500 SE

NBP2-81074MFV500

mFluor Violet 610 SE

NBP2-81074MFV610

PerCP

NBP2-81074PCP

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MNAC13

Format

Azide and BSA Free

Concentration

1 mg/ml

View all TrkA Antibodies »

Product Specifications

Immunogen

Human TrkA ECD.

Specificity

This antibody binds to the extracellular domain of TrkA. It was shown not to cross react with TrkB . Moreover MNAC13 is specific to TrkA with negligible reactivity to TrkB and TrkC.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free [NBP2-81074]

Immunocytochemistry/Immunofluorescence: TrkA Antibody (MNAC13) [NBP2-81074] - Immunofluoresence staining of fixed HeLa cells with anti-TrkA antibody (MNAC13) [NBP2-81074]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of MNAC13 [NBP2-81074] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81074, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Surface Plasmon Resonance

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human TrkA, a receptor tyrosine kinase involved in the development and the maturation of the CNS and the PNS through regulation of proliferation, differentiation, and survival of the sympathetic and nervous neurons. It is a receptor for NGF and phosphorylates several downstream proteins regulating a variety of signalling cascades. This antibody neutralizes the NGF-TrkA interation.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: TrkA

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Gene Symbol

NTRK1

Additional TrkA Products

Product Documents for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Product Datasheets

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COA

Product Specific Notices for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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