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TLR9 Antibody (26C593.2) - BSA Free

Catalog # NBP2-24729 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Canine, Equine, Monkey, Primate

Applications

Block/Neutralize, CyTOF-ready, Dot Blot, ELISA, Flow (Intracellular), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Knockdown Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 26C593.2

Format

BSA Free

Concentration

1 mg/ml

Product Summary for TLR9 Antibody (26C593.2) - BSA Free

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).

Reactivity Notes

Rhesus Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR9 Antibody (26C593.2) - BSA Free

Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY license.
Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license.

Applications for TLR9 Antibody (26C593.2) - BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 25338738)

Dot Blot

reported in scientific literature (PMID 27248820)

ELISA

1:100 - 1:2000. Use reported in multiple pieces of scientific literature

Flow (Intracellular)

1:10 - 1:1000. Use reported in scientific literature (PMID 24986635)

Functional

reported in scientific literature (PMID 25411258)

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

reported in scientific literature (PMID 27744078)

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (PMID 25871979)

In vitro assay

reported in scientific literature (PMID 25411258)

Knockdown Validated

reported in scientific literature (PMID 31655343)

Simple Western

30 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 3.8 using NBP2-24729 in the following applications:

Published Applications

Read 154 publications using NBP2-24729 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

UniProt

Product Documents for TLR9 Antibody (26C593.2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TLR9 Antibody (26C593.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for TLR9 Antibody (26C593.2) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All
  • Q: I am a bit unsure about your antibody NBP2-24729 specific against TLR9. I used it for  probing mouse B cell lysate on Proteisimple WES. On their website the recommend your antibody and claim that it detects a specific signal at 90 kDa. But on your website simple western detects a band at 200 kDa?

    A: This came during the race to 1000 and we had many people generating and reviewing data.  In retrospect, this was one that probably should not have made it out. As I reviewed the run data, it looks as though the MW ladder did not assign correctly.  When this was corrected, the band at 200 actually shifts higher, up to 280 kDa.  This band now overlaps with the 280 kDa fluorescent standard which is prone to have non-specific interactions with some antibodies.  Those two things make this non-supportive in Simple Western.   We ran Raji lysate on the same run.  This antibody detects a very broad band with a peak at ~150-165 kDa, closer to expected MW.  However, the baseline for this samples was also very high.  If we were presented with this data set today, it would not be considered supportive.  The other information on the Protein Simple web site likely came from a customer. 

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