Themis Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27492
Key Product Details
Species Reactivity
Mouse
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EERTINLPKSLKSH corresponding to C-Terminus according to NP_848781.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat Themis Antibody (NBP2-27492) is a polyclonal antibody validated for use in WB and ELISA. Anti-Themis Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Themis Antibody
![Western Blot: Themis Antibody [NBP2-27492]](https://resources.bio-techne.com/images/products/THEMIS-Antibody-Western-Blot-NBP2-27492-img0001.jpg "Western Blot: Themis Antibody [NBP2-27492]")
Western Blot: Themis Antibody [NBP2-27492]
Western Blot: THEMIS Antibody [NBP2-27492] - (0.3 ug/ml) Analysis of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Themis Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:64000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 72 kDa band observed in mouse spleen lysates (calculated MW of 72.7 kDa band according to NP_848781.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Themis
THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.
Long Name
Thymocyte-expressed Molecule Involved in Selection
Alternate Names
C6orf190, C6orf207, Gasp, SPOT, TSEPA
Entrez Gene IDs
210757 (Mouse)
Gene Symbol
THEMIS
UniProt
Additional Themis Products
Product Documents for Themis Antibody
Product Specific Notices for Themis Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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