TAG-72 Antibody (rTAG72/9132)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23722

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-23722-100ug
NBP3-23722-20ug
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rTAG72/9132

Concentration

0.2 mg/ml

View all TAG-72 Antibodies »

Product Specifications

Immunogen

Membrane-enriched fraction of a human breast carcinoma liver metastasis

Localization

Cytoplasm. Cell surface.

Specificity

Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in me

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for TAG-72 Antibody (rTAG72/9132)

TAG-72 Antibody (rTAG72/9132) Immunohistochemistry-Paraffin: TAG-72 Antibody (rTAG72/9132) [NBP3-23722] -

Immunohistochemistry-Paraffin: TAG-72 Antibody (rTAG72/9132) [NBP3-23722] -

Immunohistochemistry-Paraffin: TAG-72 Antibody (rTAG72/9132) [NBP3-23722] - Formalin-fixed, paraffin-embedded human colon stained with TAG-72 Antibody (rTAG72/9132). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for TAG-72 Antibody (rTAG72/9132)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TAG-72

Tumor-associated glycoprotein (TAG-72) is an oncofetal antigen with properties of a mucin. About 60% of carcinoma patients express TAG-72 in their sera. Majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for Ab-1 staining. Weak or no reactivity has been observed with most cell types of normal adult tissue excepting the secretory endometrium. Ab-1 is reportedly useful in distinguishing pulmonary adenocarcinomas (B72.3+) from pleural mesotheliomas (B72.3-).

Alternate Names

CA 72-4, Protein TAG-72, tag 72, tag-72 Protein TAG-72

Additional TAG-72 Products

Product Documents for TAG-72 Antibody (rTAG72/9132)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TAG-72 Antibody (rTAG72/9132)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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