T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12863
Key Product Details
Species
Human
Applications
Flow Cytometry
Product Summary for T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack
The Human T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Sampler Pack contains antibodies to detect expression of inhibitory receptors associated with T cell exhaustion. T cells co-expressing these inhibitory receptors are found during chronic viral infection and within the TME of many solid tumors. Anti-PD-1 is directly conjugated to PE, while anti- CTLA-4, TIGIT, TIM-3, VISTA/B7-H5/PD-1H and LAG-3 are directly conjugated to APC. This pack contains 6 full sized vials of directly conjugated primary antibody. It will allow the user to determine the co-expression of emerging checkpoint targets like LAG-3, TIM-3, TIGIT, and VISTA with the established checkpoint target, PD-1 using flow cytometry. All antibodies have been validted for use in flow cytometry applications.
This pack contains 1 vial each of:
FAB10863P (100 ug)
FAB3253A (100 ug)
FAB7898A (100 ug)
FAB2365RA (0.1 ml)
MAB71263APC (100 ul)
MAB23191APC (100 ul)
This pack contains 1 vial each of:
FAB10863P (100 ug)
FAB3253A (100 ug)
FAB7898A (100 ug)
FAB2365RA (0.1 ml)
MAB71263APC (100 ul)
MAB23191APC (100 ul)
Product Specifications
Clonality
Monoclonal
Specificity
Please see each vial's data sheet for specificity information specific to each product.
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Marker
T cell exhaustion
Scientific Data Images for T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack
![Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]](https://resources.bio-techne.com/images/products/T-Cell-Exhaustion-Marker-Flow-Directly-Conjugated-Flow-Cytometry-img0003.jpg "Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]")
Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]
Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.![Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]](https://resources.bio-techne.com/images/products/T-Cell-Exhaustion-Marker-Flow-Directly-Conjugated-Flow-Cytometry-img0001.jpg "Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]")
Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]
Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).![Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]](https://resources.bio-techne.com/images/products/T-Cell-Exhaustion-Marker-Flow-Directly-Conjugated-Flow-Cytometry-img0002.jpg "Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]")
Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack [NBP3-12863]
Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) - Human peripheral blood mononuclear cells (PBMCs) gated on CD3(-) cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog# FAB2408P) and either (A) Mouse Anti-Human TIGIT APC-conjugated Monoclonal Antibody (Catalog # FAB7898A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog# IC0041A).Kit Contents for T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack
Formulation, Preparation, and Storage
Formulation
See individual datasheets
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage is content dependent.
Background: T Cell Exhaustion Marker (Flow-Directly Conjugated)
Alternate Names
Immune cell exhaustion markers, immune checkpoint markers, inhibitory receptors, T cell exhaustion markers
Additional T Cell Exhaustion Marker (Flow-Directly Conjugated) Products
Product Documents for T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack
Product Specific Notices for T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...