Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7C5

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sulfotyrosine Antibodies »

Product Specifications

Immunogen

Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sulfotyrosine Antibody (7C5) - BSA Free

Western Blot: Sulfotyrosine Antibody (7C5) [NBP2-59384]

Western Blot: Sulfotyrosine Antibody (7C5) [NBP2-59384]

Western Blot: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Sulfotyrosine Antibody (7C5) [NBP2-59384]

Immunocytochemistry/ Immunofluorescence: Sulfotyrosine Antibody (7C5) [NBP2-59384]

Immunocytochemistry/Immunofluorescence: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5. Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry: Sulfotyrosine Antibody (7C5) [NBP2-59384]

Immunohistochemistry: Sulfotyrosine Antibody (7C5) [NBP2-59384]

Immunohistochemistry: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Immunohistochemistry analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Tissue: kidney. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite

Applications for Sulfotyrosine Antibody (7C5) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:25

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Sulfotyrosine

Alternate Names

sTYR;S-Tyrosine;Sulfated Tyrosine;Sulfotyrosine;Sulphated Tyrosine;Sulphotyrosine;Tyrosine sulfation;Tyrosine Sulphation

Additional Sulfotyrosine Products

Product Documents for Sulfotyrosine Antibody (7C5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sulfotyrosine Antibody (7C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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