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SSEA-5 Antibody (8e11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42922

Novus Biologicals, part of Bio-Techne
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NBP2-42922

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 8e11

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Undifferentiated human embryonic stem cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for SSEA-5 Antibody (8e11) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922]

Immunocytochemistry/ Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922]

Immunocytochemistry/Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922] - Analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.
Flow Cytometry: SSEA-5 Antibody (8e11) [NBP2-42922]

Flow Cytometry: SSEA-5 Antibody (8e11) [NBP2-42922]

Flow Cytometry: SSEA-5 Antibody (8e11) [NBP2-42922] - Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.
Immunocytochemistry/ Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922]

Immunocytochemistry/ Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922]

Immunocytochemistry/Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922] - Analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution). (A) Brightfield image (B) Dapi (C) anti-SSEA-5 Ab (D) Merged image of anti-SSEA-5 Ab and DAPI

Applications for SSEA-5 Antibody (8e11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.0763888888888889

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry

10 - 1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSEA-5

Alternate Names

SSEA5

Additional SSEA-5 Products

Product Documents for SSEA-5 Antibody (8e11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SSEA-5 Antibody (8e11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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