ssDNA Antibody (TNT-3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29849
Conjugate
Catalog #
Key Product Details
Species Reactivity
All Species
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # TNT-3
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human Raji cell nuclei.
Specificity
Reacts with single stranded DNA (ssDNA). The antibody is useful for staining nucleated cells. The antibody can also be used to identify necrotic regions of tumors and damaged tissues. By immunohistochemistry, stains heterochromatic regions of cell nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Scientific Data Images for ssDNA Antibody (TNT-3) - Azide and BSA Free
ELISA: ssDNA Antibody (TNT-3) [NBP2-29849] -
ELISA: ssDNA Antibody (TNT-3) [NBP2-29849] - Various dilutions of single stranded DNA from calf thymus (starting at 10 mg/mL) were analyzed with 0.5 mg of, Anti-DNA, single stranded, clone TNT-3. Alkaline phosphatase-conjugated Goat Anti-Mouse secondary antibody was used for detection.Applications for ssDNA Antibody (TNT-3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
5-10 ug/mL
Application Notes
ICC/IF on paraformaldehyde fixed cells Immunohistochemistry on paraformaldehyde or B5 fixed tissue sections. Flow cytometry: using 2% paraformaldehyde fixed cells. Elisa staining pattern: Stains heterochromatic regions of cell nucleus. Optimal working dilutions must be determined by end user.
See Simple Western Antibody Database for Simple Western validation: Tested in AAV purified from HEK293, separated by Charge
See Simple Western Antibody Database for Simple Western validation: Tested in AAV purified from HEK293, separated by Charge
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ssDNA
Alternate Names
Necrotic marker, Single Stranded DNA, Anti-DNA, Anti-ssDNA, Clone TNT3, Clone TNT-3, DNA Antibody, DNA antibody TNT3, ssDNA antibody, TNT-3 monoclonal Antibody
Additional ssDNA Products
Product Documents for ssDNA Antibody (TNT-3) - Azide and BSA Free
Product Specific Notices for ssDNA Antibody (TNT-3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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