Skip to main content

SR-BI Antibody

Catalog # NB400-113 | Novus Biologicals a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
NB400-113
NB400-113SS

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for SR-BI Antibody

Immunogen

Adenovirus encoding mouse SR-BI. [UniProt# Q61009]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SR-BI Antibody

Western Blot: SR-BI Antibody [NB400-113] - Detection of SR-BI (80kDa) in mouse testis lysate total protein using NB400-113.
Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-113] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-113] - Analysis of a FFPE section of human adrenal gland tissue using 1:100 dilution of SR-BI antibody. The staining was developed using HRP-DAB based detection method and the nuclei of the cells were counterstained with hematoxylin. This antibody generated a specific staining of SR-BI/SCARB1 in the glandular cells. The staining was membrane-cytoplasmic in the zona glomerulosa cells while the signal was primarily localized to the membranes of the cells in the zona fasciculata and zona reticularis layers of the adrenal cortex.

Applications for SR-BI Antibody

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 22622498)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

reported in scientific literature

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:100

Western Blot

1:500
Application Notes
This SR-BI antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot and for blocking the binding of ligands to SR-BI.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB400-113 in the following applications:

Published Applications

Read 45 publications using NB400-113 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SR-BI

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Long Name

Scavenger Receptor Class B, Member I

Alternate Names

CD36L1, CLA1, HDLQTL6, SCARB1, SR-B1, SRBI

Entrez Gene IDs

949 (Human); 20778 (Mouse)

Gene Symbol

SCARB1

Product Documents for SR-BI Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SR-BI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...

Protocols

View specific protocols for SR-BI Antibody (NB400-113):

[[URL:https://www.novusbio.com/products/sr-bi-antibody_nb400-113]][[Caption:S… Antibody]]
Blocking Protocol

1) Transfect 293 cells or Cos cells or any easily transfectable cell line with SR-BI.

2) Next day, add DMEM with 0.2% BSA to the media plus 1:500 dilution (or 1:1000) dilution of the SR-BI blocking ab. Incubate for 30 minutes to 1 hour at 37 deg C.

3) Add 1 to 10ug/ml of radiolabeled or fluorescent HDL (labeled either on the lipid or protein) to cells for 1 to 2 hours (in the presence of the blocking antibody). For control cells, do not add blocking antibody.

4) Wash cells 3 to 4 times with ice cold PBS.

5) Measure HDL uptake by appropriate method (depending on label on HDL).

Note: As a positive control, you can add an excess (100-fold more) of unlabeled HDL to cells together with the label. This should block the uptake of labeled HDL by 80% or more. This positive control should tell you that your cells are expressing functional SR-BI. Also, cells not receiving either unlabeled HDL or no blocking ab should tell you that your cells are expressing functional SR-BI.
[[URL:https://www.novusbio.com/products/sr-bi-antibody_nb400-113]][[Caption:S… Antibody]]
Immunocytochemistry Protocol

Culture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 5-10 minutes.
2. Remove the formalin and add 0.5% Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
3. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1% Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
4. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
5. To block nonspecific antibody binding incubate in 10% normal goat serum for 1 hour at room temperature.
6. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
7. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
8. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
10. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 ug/ml, or coverslips can be directly mounted in media containing DAPI.
11. Cells can now be viewed with a fluorescence microscope.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.

[[URL:https://www.novusbio.com/products/sr-bi-antibody_nb400-113]][[Caption:S… Antibody]]
Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 30 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute anti-SR-BI rabbit primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

FAQs for SR-BI Antibody

Showing  1 - 5 of 5 FAQs Showing All
  • Q: Do you have information on epitope of NB400-113?

    A: NB400-113 is not epitope mapped and was generating by using an immunogen of full-length mouse SR-BI expressed by adenovirus

  • Q: Is this Ab identical to the former Ab3811 - Ab 3811 was used by Liisanantti et al. (Alcoholism: Clinical and Experimental Research 2009) is the Ab3811 still available?

    A: Yes, Ab3811 from Abcam and the antibody NB400-113 from Novus are one and the same. The Abcam catalog number is our antibody that they are distributing. NB400-113 should perform as indicated in the literature under Ab3811.

  • Q: Could you please give me information about the molecular weight of SR-BI-antibody (NB400-113)?

    A: Encoded by gene SCARB1, SR-BI is a plasma membrane receptor for high density lipoprotein cholesterol (HDL) and its expected molecular weight is 61kD. However, this protein undergoes post-translational modifications including N-glycosylation which causes a significant increase in the molecular mass from 61kD to up to approximately 82kD.

  • Q: We are interested in using this antibody to perform staining on cells and to detect cells with FACS (flow cytometry). It is indicated on the datasheet that it is possible to use this antibody for flow cytometry, however little information is provided. Could you please inform us on the isotype of the antibody and the concentration we need to use for our FACS experiments?

    A: This antibody is a rabbit polyclonal, so the isotype is IgG. We have never tested in-house for flow cytometry. This antibody has been validated for use in Flow Cytometry by other researchers in publications. All the information we have regarding its use in flow cytometry comes from the publications on the datasheet (PMID: 20041149 and 22622498). We recommend 1:50-1:200 for ICC, so this is probably a good range to start your testing with.

  • Q: Please provide an estimated concentration for NB400-113 Lot G7?

    A: As mentioned on the datasheet, this product is an unpurified formulation (whole antiserum) and the exact concentration of antibody is not quantifiable.
    However, the concentration of antibody in antisera can vary from 1-10 mg/ml.

Showing  1 - 5 of 5 FAQs Showing All
Loading...