Smith Antigen Antibody (Y12)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-546
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Drosophila, Rabbit
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunomicroscopy, Western Blot
Cited:
ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # Y12
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen is generated from human Smith Antigen.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 17473171). Canine (PMID: 10330151). Mouse reactivity reported in scientific literature (PMID: 28093526).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Nuclear
Specificity
Ab-1 recognizes cross-reactive epitopes on the B'/B and D polypeptides of Sm. Ab-1 precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Scientific Data Images for Smith Antigen Antibody (Y12)
![Western Blot: Smith Antigen Antibody (Y12) [NB600-546]](https://resources.bio-techne.com/images/products/Smith-Antigen-Antibody-Y12-Western-Blot-NB600-546-img0006.jpg "Western Blot: Smith Antigen Antibody (Y12) [NB600-546]")
Western Blot: Smith Antigen Antibody (Y12) [NB600-546]
Western Blot: Smith Antigen Antibody (Y12) [NB600-546] - Analysis of LS174T Cells.![Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546]](https://resources.bio-techne.com/images/products/Smith-Antigen-Antibody-Y12-Immunohistochemistry-Paraffin-NB600-546-img0005.jpg "Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546]")
Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546]
Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546] - Human tonsil stained with Smith antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.Applications for Smith Antigen Antibody (Y12)
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:50 - 1:100
Immunomicroscopy
1:10 - 1:500
Western Blot
0.5 - 1.0 ug/ml
Application Notes
WB: Detects an approx. 25 kDa, which corresponds to the predicted molecular weight of Smith Antigen.Precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all of these RNA-protein complexes. Use in ELISA reported in scientific literature (PMID 28093526). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28515487).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Smith Antigen
Alternate Names
Smith antigen
Additional Smith Antigen Products
Product Documents for Smith Antigen Antibody (Y12)
Product Specific Notices for Smith Antigen Antibody (Y12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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