I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?

My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.

My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.

We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

SMC1 Antibody - BSA Free

Name: SMC1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP3-29588

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29588

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMC1 Antibodies »

Product Specifications

Immunogen

between 1175 and C-term

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SMC1 Antibody - BSA Free (NBP3-29588) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SMC1 Antibody - BSA Free

Immunohistochemistry;SMC1 Antibody[NBP3-29562]

Immunohistochemistry;SMC1 Antibody[NBP3-29562]Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 . Detection: DAB

Immunohistochemistry;SMC1 Antibody[NBP3-29588]

Immunohistochemistry;SMC1 Antibody[NBP3-29588]Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 . Detection: DAB

Immunoprecipitation:SMC1 AntibodyNBP3-29588] -

Immunoprecipitation:SMC1 AntibodyNBP3-29588] -Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody NBP3-29588 (lot NBP3-29588-6) used for IP at 6 µg per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018). For blotting immunoprecipitated SMC1, A700-018 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.

Applications for SMC1 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC1

SMC1 is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of the cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, SMC1 works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.

Long Name

Structural Maintenance of Chromosomes 1

Alternate Names

SB1.8, SMC1A, SMC1L1

Gene Symbol

SMC1A

Additional SMC1 Products

Product Documents for SMC1 Antibody - BSA Free

Product Datasheets

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COA

Product Specific Notices for SMC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SMC1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29588

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all SMC1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Scientific Data Images for SMC1 Antibody - BSA Free

Immunohistochemistry;SMC1 Antibody[NBP3-29562]

Immunohistochemistry;SMC1 Antibody[NBP3-29588]

Immunoprecipitation:SMC1 AntibodyNBP3-29588] -

Applications for SMC1 Antibody - BSA Free

Immunohistochemistry

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: SMC1

Long Name

Alternate Names

Gene Symbol

Additional SMC1 Products

Product Documents for SMC1 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for SMC1 Antibody - BSA Free