Sleeping Beauty Transposase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2798
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 324622
Product Specifications
Immunogen
E. coli-derived recombinant Sleeping Beauty Transposase
Specificity
Detects Sleeping Beauty (SB) Transposase in Western blots. In Immunohistochemistry tests, this antibody stains SB transposase transgenic mouse tissue sections but not wild type mouse tissue sections.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Sleeping Beauty Transposase Antibody
Sleeping Beauty Transposase in Mouse Intestine.
Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of intestine from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Sleeping Beauty Transposase in Mouse Liver.
Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of liver from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Sleeping Beauty Transposase Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse intestine and liver, both subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Perfusion fixed paraffin-embedded sections of mouse intestine and liver, both subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: Recombinant Sleeping Beauty Transposase
Sample: Recombinant Sleeping Beauty Transposase
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sleeping Beauty Transposase
References
- Ivics, Z. et al. (1997) Cell 91:501.
Additional Sleeping Beauty Transposase Products
Product Documents for Sleeping Beauty Transposase Antibody
Product Specific Notices for Sleeping Beauty Transposase Antibody
For research use only
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