Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SCAP Antibodies »

Product Specifications

Immunogen

SREBF chaperone. (Uniprot# Q12770)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCAP Antibody - BSA Free

SCAP Antibody - BSA Free

Immunohistochemistry: SCAP Antibody - BSA Free [NBP3-45439] -

Immunohistochemistry: SCAP Antibody - BSA Free [NBP3-45439] - Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (SCAP Antibody) at dilution of 1:50
SCAP Antibody - BSA Free

Western Blot: SCAP Antibody - BSA Free [NBP3-45439] -

Western Blot: SCAP Antibody - BSA Free [NBP3-45439] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (SCAP antibody) at dilution of 1:300

Applications for SCAP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:20-1:200

Western Blot

1:500-1:2000
Application Notes
Theoretical Molecular Weight is 140 kDa, 96 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCAP

Sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) is a molecular chaperone that plays a role in the biosynthesis of cholesterol and triglycerides (1). SCAP is a membrane protein that helps shuttle SREBP from the endoplasmic reticulum (ER) via coat protein II (COPII) vesicles to the golgi where SREBP is cleaved via protease S1P and S2P, becomes activated, and is translocated to the nucleus for transcription (1-3). The SCAP protein has a theoretical molecular weight of 140 kDa and is comprised of 1276 amino acids with an N-terminal domain containing 8 transmembrane alpha helices, where transmembrane 2-6 consist of a sterol-sensing domain, and a C-terminal domain with WD40 motif repeats (1,3). The SCAP-SREBP association plays a pivotal role in regulating lipid metabolism and lipogenesis (1,4). SREBP activation via SCAP is associated with accumulation of triglycerides and cholesterol leading to metabolic disorders including diabetes, atherosclerosis, and nonalcoholic fatty liver disease (NAFLD) (1,4). Animal models have revealed that inhibition or blocking of SCAP using antagonists may be a potential therapeutic target for hyperlipidemia and hypertriglyceridemia (1,4).

References

1. Lee, S. H., Lee, J. H., & Im, S. S. (2020). The cellular function of SCAP in metabolic signaling. Experimental & molecular medicine, 52(5), 724-729. https://doi.org/10.1038/s12276-020-0430-0

2. Cheng, X., Li, J., & Guo, D. (2018). SCAP/SREBPs are Central Players in Lipid Metabolism and Novel Metabolic Targets in Cancer Therapy. Current topics in medicinal chemistry, 18(6), 484-493. https://doi.org/10.2174/1568026618666180523104541

3. Brown, M. S., Radhakrishnan, A., & Goldstein, J. L. (2018). Retrospective on Cholesterol Homeostasis: The Central Role of Scap. Annual review of biochemistry, 87, 783-807. https://doi.org/10.1146/annurev-biochem-062917-011852

4. Moon Y. A. (2017). The SCAP/SREBP Pathway: A Mediator of Hepatic Steatosis. Endocrinology and metabolism (Seoul, Korea), 32(1), 6-10. https://doi.org/10.3803/EnM.2017.32.1.6

Alternate Names

KIAA0199, PSEC0227, SREBF chaperone, SREBP cleavage-activating protein, sterol regulatory element binding protein cleavage-activating protein

Gene Symbol

SCAP

Additional SCAP Products

Product Documents for SCAP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SCAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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