SATB2 Antibody (CL0323) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52916
![Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0323) [NBP2-52916]](https://resources.bio-techne.com/images/products/SATB2-Antibody-CL0323-Immunocytochemistry-Immunofluorescence-NBP2-52916-img0007.jpg "Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0323) [NBP2-52916]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0323
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q9UPW6, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse SATB2 Antibody (CL0323) - BSA Free (NBP2-52916) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SATB2 Antibody (CL0323) - BSA Free
Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0323) [NBP2-52916]
Immunocytochemistry/Immunofluorescence: SATB2 Antibody (CL0323) [NBP2-52916] - Staining of U-2 OS cells using the Anti-SATB2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Western Blot: SATB2 Antibody (CL0323) [NBP2-52916]](https://resources.bio-techne.com/images/products/SATB2-Antibody-CL0323-Western-Blot-NBP2-52916-img0006.jpg "Western Blot: SATB2 Antibody (CL0323) [NBP2-52916]")
Western Blot: SATB2 Antibody (CL0323) [NBP2-52916]
Western Blot: SATB2 Antibody (CL0323) [NBP2-52916] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: SATB2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)![Western Blot: SATB2 Antibody (CL0323) [NBP2-52916]](https://resources.bio-techne.com/images/products/SATB2-Antibody-CL0323-Western-Blot-NBP2-52916-img0005.jpg "Western Blot: SATB2 Antibody (CL0323) [NBP2-52916]")
Western Blot: SATB2 Antibody (CL0323) [NBP2-52916]
Western Blot: SATB2 Antibody (CL0323) [NBP2-52916] - Lane 1: Marker [kDa], Lane 2: Human cell line HEL
Applications for SATB2 Antibody (CL0323) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SATB2
Alternate Names
DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2
Gene Symbol
SATB2
Additional SATB2 Products
Product Documents for SATB2 Antibody (CL0323) - BSA Free
Product Specific Notices for SATB2 Antibody (CL0323) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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