SARS-CoV-2 ORF6 Antibody - BSA Free

Western Blot: SARS-CoV-2 ORF6 Antibody - BSA Free [NBP3-05707] -

ORF6 does not antagonize IFN production. (A–E) Calu-3 cells were uninfected or infected with SARS-CoV-2 WT virus or deltaORF6 virus (MOI of 0.5). At 24 hpi, cells were treated with Sendai virus (SeV) for 8 h as indicated. (A and B) Total RNA was extracted, and induction of IFN-beta (A) and IFN-lambda 1 (B) was examined by RT-qPCR. Shown is the mean +/- SEM for three independent experiments. The significance was calculated using Kruskal-Wallis test and is indicated by *P < 0.05 (ns, not significant). (C) Cell lysates were collected, and the protein expression level was determined by immunoblotting using indicated antibodies. Shown are the representative blots of two independent experiments. (D) Cells were fixed and stained with antibodies against IRF3 and SARS-CoV-2 Spike. Representative images of two independent experiments are shown. (E) Percent of IRF3 translocation in virally infected cells was quantified. Shown is the average percent for six fields of view from two independent experiments. Significance was calculated using an unpaired, two-tailed Student’s t test (ns, not significant). (F through H) Calu-3 cells were infected with SARS-CoV-2 WT virus or deltaORF6 virus (MOI of 0.5) for 24 h, followed by treatment of Poly(I:C) for 6 h as indicated. (F and G) mRNA expression of IFN-beta (F) and IFN-lambda 1 (G) was determined by RT-qPCR and normalized to uninfected cells. Shown are the means +/- SEM for three independent experiments. The significance was calculated using Kruskal-Wallis test (ns, not significant). (H) Immunoblotting was performed to monitor protein expression using indicated antibodies. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37377442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SARS-CoV-2 ORF6 Antibody - BSA Free [NBP3-05707] -

ORF6 does not antagonize IFN production. (A–E) Calu-3 cells were uninfected or infected with SARS-CoV-2 WT virus or deltaORF6 virus (MOI of 0.5). At 24 hpi, cells were treated with Sendai virus (SeV) for 8 h as indicated. (A and B) Total RNA was extracted, and induction of IFN-beta (A) and IFN-lambda 1 (B) was examined by RT-qPCR. Shown is the mean +/- SEM for three independent experiments. The significance was calculated using Kruskal-Wallis test and is indicated by *P < 0.05 (ns, not significant). (C) Cell lysates were collected, and the protein expression level was determined by immunoblotting using indicated antibodies. Shown are the representative blots of two independent experiments. (D) Cells were fixed and stained with antibodies against IRF3 and SARS-CoV-2 Spike. Representative images of two independent experiments are shown. (E) Percent of IRF3 translocation in virally infected cells was quantified. Shown is the average percent for six fields of view from two independent experiments. Significance was calculated using an unpaired, two-tailed Student’s t test (ns, not significant). (F through H) Calu-3 cells were infected with SARS-CoV-2 WT virus or deltaORF6 virus (MOI of 0.5) for 24 h, followed by treatment of Poly(I:C) for 6 h as indicated. (F and G) mRNA expression of IFN-beta (F) and IFN-lambda 1 (G) was determined by RT-qPCR and normalized to uninfected cells. Shown are the means +/- SEM for three independent experiments. The significance was calculated using Kruskal-Wallis test (ns, not significant). (H) Immunoblotting was performed to monitor protein expression using indicated antibodies. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37377442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SARS-CoV-2 ORF6 Antibody - BSA Free [NBP3-05707] -

SARS-CoV-2 induces immune responses in bystander cells. (A) Calu-3 cells were either uninfected (Uninf.) or infected with SARS-CoV-2 WT virus or deltaORF6 virus (MOI = 0.5) for 24, 36, or 48 h. Cells were lysed, and the protein expression level was determined by immunoblotting using indicated antibodies. Shown are representative blots of two independent experiments. (B through E) Calu-3 cells were either uninfected or infected with SARS-CoV-2 WT virus or deltaORF6 virus (MOI of 0.5) for 48 h. Cells were fixed and stained with antibodies against IRF3 (B) or phospho-STAT1 (p-Stat1) (D). Quantification of IRF3 nuclear translocation (C) in panel (B) or p-Stat1 nuclear translocation (E) in panel (D). Graphs show the average percent of nuclear translocation in viral-infected cells or bystander cells for six fields of view collected from two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37377442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.