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SARS-CoV-2 nsp6 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16000

Novus Biologicals, part of Bio-Techne
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NBP3-16000-100ul
NBP3-16000-20ul

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 236-290 of coronavirus NSP6 (YP_009725302.1). RLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

794 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SARS-CoV-2 nsp6 Antibody - Azide and BSA Free

Western Blot: SARS-CoV-2 nsp6 AntibodyAzide and BSA Free [NBP3-16000]

Western Blot: SARS-CoV-2 nsp6 AntibodyAzide and BSA Free [NBP3-16000]

Western Blot: SARS-CoV-2 nsp6 Antibody [NBP3-16000] - Analysis of extracts of normal 293T cells and 293T transfected with NSP6 Protein, using SARS-CoV-2 nsp6 antibody (NBP3-16000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for SARS-CoV-2 nsp6 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp6

SARS-CoV-2 Nonstructural Protein 6 (NSP6) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP6 is 290 amino acids (aa) with a theoretical molecular weight of 33 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP6 has 88.2% sequence identity and 98.3% sequence similarity (1). Structurally, NSP6 is a transmembrane protein with six transmembrane domains (3). SARS-CoV-2 NSP6 functions in autophagosome formation and double membrane vesicle formation (1,3). A SARS-CoV-2 interactome study revealed that NSP6 has a role in vesicle trafficking. Specifically, it is shown to interact with vacuolar ATPase (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

Alternate Names

Non-structural protein 6

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp6 Products

Product Documents for SARS-CoV-2 nsp6 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp6 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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