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SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25656

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25656

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1445 expressed in HEK293

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 nsp15. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free (NBP3-25656) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free [NBP3-25656] -

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free [NBP3-25656] - SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] detects SARS-CoV-2 (COVID-19) nsp15 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp15 stained by SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (NBP3-25656) diluted at 1:1000. Blue: Fluoroshield with DAPI .
SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

Immunohistochemistry-Paraffin: SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free [NBP3-25656] -

Immunohistochemistry-Paraffin: SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free [NBP3-25656] - SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] detects SARS-CoV-2 (COVID-19) nsp15 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) nsp15 transfected 293T. Green: SARS-CoV-2 (COVID-19) nsp15 stained by SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (NBP3-25656) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

Western Blot: SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free [NBP3-25656] -

Western Blot: SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free [NBP3-25656] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (NBP3-25656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp15

Alternate Names

endoRNAse, Non-structural protein 15

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp15 Products

Product Documents for SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp15 Antibody (HL1445) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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