SARS-CoV-2 nsp13 Antibody [mFluor Violet 610 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07056MFV610

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA, Immunohistochemistry

Label

mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 nsp13 Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 14 amino acids near the center of the SARS-CoV-2 (COVID-19) NSP13 (Helicase) protein. The immunogen is located between 440-490 amino acids of SARS-CoV-2 (COVID-19) NSP13 (Helicase).

Reactivity Notes

Predicted reactivity based on immunogen sequence: SARS-CoV NSP13 (Helicase) protein: (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for SARS-CoV-2 nsp13 Antibody [mFluor Violet 610 SE]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SARS-CoV-2 nsp13

SARS-CoV-2 Nonstructural Protein 13 (NSP13) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP13 is 601 amino acids (aa) with a theoretical molecular weight of 66.9 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP13 has 99.8% sequence identity and 100% sequence similarity, with only one amino acid residue difference between the two proteins (1). NSP13 is suggested to have helicase activity and unwinds RNA (1,3). Furthermore, NSP13 is suggested to interact with NSP12 and through protein-protein interactions, NSP12 enhances NSP13's helicase activity (1,3,4). Additionally, a SARS-CoV-2 interactome study reveals NSP13's involvement in several processes including transcriptional regulation, Golgi organization, protein kinase A signaling, and centrosome complex formation (2). Given its role in centrosome organization, NSP13 is a possible molecular link for the loss of smell, called anosmia, due to the olfactory cilia defects that can occur because of COVID-19 infection (5). NSP13 also interacts with innate immune pathways including the interferon pathway and NF-kappaB pathway (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

5. Li, W., Li, M., & Ou, G. (2020). COVID-19, cilia, and smell. The FEBS journal. https://doi.org/10.1111/febs.15491

Alternate Names

helicase, Non-structural protein 13

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp13 Products

Product Documents for SARS-CoV-2 nsp13 Antibody [mFluor Violet 610 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 nsp13 Antibody [mFluor Violet 610 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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