Sarcosine oxidase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-649
![Western Blot: Sarcosine oxidase Antibody [NB600-649]](https://resources.bio-techne.com/images/products/Sarcosine oxidase Antibody-Western Blot-NB600-649-img0007.jpg "Western Blot: Sarcosine oxidase Antibody [NB600-649]")
Key Product Details
Species Reactivity
Bacteria
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Sarcosine oxidase [Microbial] (Uniprot: Q46337)
Reactivity Notes
Cross reactivity against Sarcosine oxidase from other sources is unknown.
Specificity
Cross reactivity against Sarcosine oxidase from other sources is unknown.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Sarcosine oxidase Antibody - BSA Free
Western Blot: Sarcosine oxidase Antibody [NB600-649]
Western Blot: Sarcosine oxidase Antibody [NB600-649] - Detection of Sarcosine Oxidase. Samples of 1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Sarcosine Oxidase. Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat 1:10K 1.5 hr RT in and imaged on the VersaDoc imaging system.![Western Blot: Sarcosine oxidase Antibody [NB600-649]](https://resources.bio-techne.com/images/products/Sarcosine-oxidase-Antibody-Western-Blot-NB600-649-img0006.jpg "Western Blot: Sarcosine oxidase Antibody [NB600-649]")
Western Blot: Sarcosine oxidase Antibody [NB600-649]
Western Blot: Sarcosine oxidase Antibody [NB600-649] - Analysis using HRP conjugate of NB600-649. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.Applications for Sarcosine oxidase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by dot blot and western blot and is suitable for ELISA assay against 1.0 ug of Sarcosine Oxidase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Sarcosine oxidase
UniProt
Additional Sarcosine oxidase Products
Product Documents for Sarcosine oxidase Antibody - BSA Free
Product Specific Notices for Sarcosine oxidase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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