RSK1 Antibody (E4)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-57469

Discontinued Product
NB110-57469 has been discontinued. View all RSK1 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # E4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RSK1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to residues in N-terminus of human p90-RSK1 was used as immunogen.

Reactivity Notes

Reacts with: Mouse, Rat, Human

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for RSK1 Antibody (E4)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10

Western Blot

1:1000
Application Notes
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 83kDa.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

49% PBS, 0.05% BSA and 50% Glycerol

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RSK1

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Entrez Gene IDs

6195 (Human); 20111 (Mouse); 81771 (Rat)

Gene Symbol

RPS6KA1

UniProt

Q15418

Additional RSK1 Products

Product Documents for RSK1 Antibody (E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RSK1 Antibody (E4)

Produced using Abcam's RabMab&#174 technology. RabMab&#174 technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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