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RFP Antibody

Catalog # NBP1-97371 | Novus Biologicals a Bio-Techne Brand
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NBP1-97371

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for RFP Antibody

Immunogen

The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. (Uniprot: Q9U6Y8)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30159312).

Specificity

RFP Antibody was tested by western blot.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Description

Chicken products cannot be exported to Canada.



RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for RFP Antibody

Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.
Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]
Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]

Applications for RFP Antibody

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:1000-1:3000
Application Notes
This product is designed to detect recombinant RFP. This product antibody has been tested by ELISA, SDS-PAGE, and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 30159312).

Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30158698). Use in IHC-P reported in scientific literature (PMID:34389674).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 10 publications using NBP1-97371 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: RFP

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Alternate Names

DSRED, GFP-like chromoprotein, Red Fluorescent Protein, RFP

Product Documents for RFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFP Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for RFP Antibody

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  • Q: Would this antibody detect DsRed?

    A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

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