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RFP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69962

Novus Biologicals, part of Bio-Techne
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NBP1-69962

Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Reactivity Notes

No reaction was observed against Human, Mouse or Rat serum proteins

Specificity

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. No reaction was observed against Human, Mouse or Rat serum proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma)

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for RFP Antibody - BSA Free

Western Blot: RFP Antibody [NBP1-69962]

Western Blot: RFP Antibody [NBP1-69962]

Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Immunocytochemistry/ Immunofluorescence: RFP Antibody [NBP1-69962]

Immunocytochemistry/ Immunofluorescence: RFP Antibody [NBP1-69962]

Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Immunofluorescence Microscopy of RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue.Fixation: 4% PFA. Antigen retrieval: Heat.Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT.Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT.Staining: cardiac cells are RFP positive in red in tomato transgenic mice.
Immunohistochemistry: RFP Antibody [NBP1-69962]

Immunohistochemistry: RFP Antibody [NBP1-69962]

Immunohistochemistry: RFP Antibody [NBP1-69962] - Immunohistochemistry of RFP antibody.Tissue: Mouse gut tissue in tomato transgenic mice.Fixation: formalin fixed paraffin embedded. Antigen retrieval: heat 5 min at high temp in 1X rodent decloaker. Primary antibody: RFP antibody at 1:200 for 1 h 30 min at RT.Secondary Antibody: HRP anti-rabbit.

Applications for RFP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:28700-1:48700

Flow Cytometry

1:200-1:2000

Immunocytochemistry/ Immunofluorescence

1:200-1:2000

Immunohistochemistry

1:200-1:2000

Simple Western

1:20

Western Blot

1:1000-1:5000
Application Notes
This product is designed to detect RFP and its variants. This antibody has been tested by ELISA, western blot, IF, and IHC, and is suitable for use in IP, ICC, dual RNA-FISH, iDISCO+, IEM, and FLOW. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

RFP Antibody validated for CyTOF-ready from a verified customer review.

Reviewed Applications

Read 1 review rated 3 using NBP1-69962 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: RFP

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

Alternate Names

DSRED, GFP-like chromoprotein, Red Fluorescent Protein, RFP

Additional RFP Products

Product Documents for RFP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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