RASAL3 Antibody - BSA Free

Western Blot: RASAL3 Antibody [NBP2-83439]

Western Blot: RASAL3 Antibody [NBP2-83439] - Host: Rabbit. Target Name: RASAL3. Sample Tissue: Human Jurkat Whole Cell lysates. Antibody Dilution: 1ug/ml

Western Blot: RASAL3 Antibody - BSA Free [NBP2-83439] -

ALKBH5 exerts cardioprotective effects by promoting the Ras/Raf/Erk signaling pathway via m6A demethylation of Rasal3 mRNA(A) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KO-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown.(B) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KO-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(C) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KO-CM-DOX and WT-CM-DOX after OE-Rasal3 overexpression.(D) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KO-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(E) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KI-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown.(F) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KI-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(G) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KI-CM-DOX and WT-CM-DOX after OE-Rasal3 overexpression.(H) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KI-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(I) A proposed model showing how ALKBH5 mediates mitochondrial dysfunction to induce CM death and mitigate DIC injury. Data are depicted as the mean +/- SEM. Statistical significance was determined by Student’s t test or one-way ANOVA or two-way ANOVA with a post-hoc Holm-Sidak test. Here, ns, not significant; ∗p < 0.05; ∗∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36876119), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL3 Antibody - BSA Free [NBP2-83439] -

ALKBH5 exerts cardioprotective effects by promoting the Ras/Raf/Erk signaling pathway via m6A demethylation of Rasal3 mRNA(A) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KO-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown.(B) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KO-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(C) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KO-CM-DOX and WT-CM-DOX after OE-Rasal3 overexpression.(D) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KO-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(E) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KI-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown.(F) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KI-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(G) Representative western blots of Rasal3, Ras-GTP (active Ras), phosphorylated cRaf (phos-cRaf), and phosphorylated Erk (phos-Erk1/2) in ALKBH5-KI-CM-DOX and WT-CM-DOX after OE-Rasal3 overexpression.(H) Western blot analysis of Rasal3 and Ras-GTP in ALKBH5-KI-CM-DOX and WT-CM-DOX after shRNA-Rasal3 knockdown (n = 3).(I) A proposed model showing how ALKBH5 mediates mitochondrial dysfunction to induce CM death and mitigate DIC injury. Data are depicted as the mean +/- SEM. Statistical significance was determined by Student’s t test or one-way ANOVA or two-way ANOVA with a post-hoc Holm-Sidak test. Here, ns, not significant; ∗p < 0.05; ∗∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36876119), licensed under a CC-BY license. Not internally tested by Novus Biologicals.