RAB14 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84720
![Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]](https://resources.bio-techne.com/images/products/RAB14-Antibody-Immunohistochemistry-NBP1-84720-img0011.jpg "Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RAB14 Antibody - BSA Free
Western Blot Shows RAB14 Specificity Using Knockdown Cell Line.
Western blot shows lysates of DMS 53 parental cell line and RAB14 knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with RAB14 Antibody (Catalog # NBP1-84720) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB14 at approximately 23.8 kDa (as indicated) in the parental DMS 53 cell line but is significantly reduced in knockdown DMS 53 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.![Immunocytochemistry/ Immunofluorescence: RAB14 Antibody [NBP1-84720]](https://resources.bio-techne.com/images/products/RAB14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84720-img0008.jpg "Immunocytochemistry/ Immunofluorescence: RAB14 Antibody [NBP1-84720]")
Immunocytochemistry/ Immunofluorescence: RAB14 Antibody [NBP1-84720]
Immunocytochemistry/Immunofluorescence: RAB14 Antibody [NBP1-84720] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.![Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]](https://resources.bio-techne.com/images/products/RAB14-Antibody-Immunohistochemistry-Paraffin-NBP1-84720-img0014.jpg "Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]")
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]](https://resources.bio-techne.com/images/products/RAB14-Antibody-Immunohistochemistry-Paraffin-NBP1-84720-img0012.jpg "Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]")
![Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]](https://resources.bio-techne.com/images/products/RAB14-Antibody-Immunohistochemistry-Paraffin-NBP1-84720-img0013.jpg "Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]")
![RAB14 Antibody - BSA Free Western Blot: RAB14 Antibody - BSA Free [NBP1-84720]](https://resources.bio-techne.com/images/products/nbp1-84720_rabbit-polyclonal-rab14-antibody-10420251540310.jpg "Western Blot: RAB14 Antibody - BSA Free [NBP1-84720]")
Applications for RAB14 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RAB14
Alternate Names
bA165P4.3 (member RAS oncogene family), F protein-binding protein 1, FBP, RAB-14, RAB14, member RAS oncogene family, ras-related protein Rab-14, small GTP binding protein RAB14
Gene Symbol
RAB14
Additional RAB14 Products
Product Documents for RAB14 Antibody - BSA Free
Product Specific Notices for RAB14 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...