Pyranose Oxidase Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77709
Key Product Details
Species Reactivity
E. coli
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Pyranose Oxidase [E.coli] (Uniprot: P79076)
Reactivity Notes
Cross reactivity against Pyranose Oxidase from other sources is unknown.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli]
Scientific Data Images for Pyranose Oxidase Antibody [Biotin]
Western Blot: Acetylcholinesterase/ACHE Antibody [Biotin] [NBP1-77709] - Goat anti Pyranose Oxidase antibody was used to detect pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat 1:10K in TBS 1 hr RT.
Applications for Pyranose Oxidase Antibody [Biotin]
Application
Recommended Usage
ELISA
1:5000 - 1:20000
Immunoprecipitation
1:100
Western Blot
1:500 - 1:5000
Application Notes
This product has been assayed against 1.0 ug of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:70,000 to 1:350,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Pyranose Oxidase
Alternate Names
pyranose 2-oxidase
Additional Pyranose Oxidase Products
Product Documents for Pyranose Oxidase Antibody [Biotin]
Product Specific Notices for Pyranose Oxidase Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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