PRPF8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22273
![Western Blot: PRPF8 Antibody [NBP2-22273]](https://resources.bio-techne.com/images/products/PRPF8-Antibody-Western-Blot-NBP2-22273-img0001.jpg "Western Blot: PRPF8 Antibody [NBP2-22273]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1825 and 1875 of human Pre-mRNA-Processing Factor 8 using the numbering given in entry NP_006436.3 (GeneID 10594).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PRPF8 Antibody - BSA Free
![Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273]](https://resources.bio-techne.com/images/products/PRPF8-Antibody-Immunohistochemistry-NBP2-22273-img0005.jpg "Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273]")
Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273]
Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273] - Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- PRPF8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: PRPF8 Antibody [NBP2-22273]](https://resources.bio-techne.com/images/products/PRPF8-Antibody-Immunoprecipitation-NBP2-22273-img0002.jpg "Immunoprecipitation: PRPF8 Antibody [NBP2-22273]")
Immunoprecipitation: PRPF8 Antibody [NBP2-22273]
Immunoprecipitation: PRPF8 Antibody [NBP2-22273] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22273 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22274 For blotting immunoprecipitated PRPF8, NBP2-22273 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for PRPF8 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PRPF8
Alternate Names
apoptosis-regulated protein 1, apoptosis-regulated protein 2, p220, precursor mRNA processing protein, pre-mRNA-processing-splicing factor 8, Prp8, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae), PRP8 pre-mRNA processing factor 8 homolog (yeast), PRP8,220 kDa U5 snRNP-specific protein, PRPC8HPRP8, RP13, Splicing factor Prp8, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p
Gene Symbol
PRPF8
UniProt
Additional PRPF8 Products
Product Documents for PRPF8 Antibody - BSA Free
Product Specific Notices for PRPF8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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