phycoerythrin Antibody (PE-DLF) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00145
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PE-DLF
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody is Phycoerythrin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for phycoerythrin Antibody (PE-DLF) [Biotin]
Flow Cytometry: phycoerythrin Antibody (PE-DLF) [Biotin] [NBP2-00145] - Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (B220) PE and 0.06 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Phycoerythrin Biotin (right) followed by Streptavidin APC . Total viable cells were used for analysis.
Applications for phycoerythrin Antibody (PE-DLF) [Biotin]
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Application Notes
This PE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: phycoerythrin
Alternate Names
PE, R-PE
Additional phycoerythrin Products
Product Documents for phycoerythrin Antibody (PE-DLF) [Biotin]
Product Specific Notices for phycoerythrin Antibody (PE-DLF) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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