Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody

Name: Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: IC37173R

R&D Systems, part of Bio-Techne | Catalog # IC37173R

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

Discontinued Product

IC37173R has been discontinued. View all STAT6 products.

Key Product Details

Species Reactivity:	Human
Immunogen:	Phosphopeptide containing human STAT6 Y641 site. Accession # P42226
Applications:	Flow Cytometry
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Source:	Recombinant Monoclonal Rabbit IgG Clone # 1248D
Specificity:	Detects human STAT6 when phosphorylated at Y641.

View Full Technical Details for Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	5 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples

	Intracellular Staining by Flow Cytometry Detection of Phospho-STAT6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC37173R) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC1051R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
	Intracellular Staining by Flow Cytometry Detection of Phospho-STAT6 in Human HeLa cell line by Flow Cytometry. HeLa human cervical endothelial carcinoma cell line treated with recombinant human IL-4 (204-IL; 100 ng/mL) for 15 minutes was stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC37173R, filled histogram) or Rabbit IgG control antibody (IC1051R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with methanol. Staining was performed using our Staining Intracellular Molecules protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN₄GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Item	Value
Long Name	Signal Transducer and Activator of Transcription 6
Entrez Gene IDs	6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names	D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6, STAT6B, STAT6C, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, transcription factor IL-4 STAT

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC37173R

Key Product Details

Applications

Scientific Data Examples

Intracellular Staining by Flow Cytometry

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: STAT6

Product Specific Notices

Customer Reviews

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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