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Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

Catalog # NBP2-78792 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # MECA-79R

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

Immunogen

Mouse lymph node stromal cells

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Description

Clone MECA-79R (rat IgG1) is a recombinant version of the original clone MECA-79 (rat IgM).

Scientific Data Images for Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

Western Blot: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Analysis of FFPE mouse adipose tissue section (with lymph node areas) using Peripheral Node Addressin antibody (clone MECA-79) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Peripheral Node Addressin antibody generated a strong and specific staining of MECA-79 antigen in the the cytoplasm and the membranes of high endothelial venules (HEVs) aka peripheral lymph node addressin (PNAd) in lymph node areas of tested adipose tissue section.
Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Analysis of FFPE human tonsil tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.

Applications for Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31278331)

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

In vivo assay

reported in scientific literature (PMID 30277476)

Western Blot

1:100-1:2000
Application Notes
Additional reported application of in vitro and in vivo blocking of cell adhesion.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 24 publications using NBP2-78792 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 °C.

Background: Peripheral Node Addressin

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Alternate Names

MECA-79, Peripheral Node Addressin, PNAd

Product Documents for Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Peripheral Node Addressin Antibody (MECA-79R) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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