Papain Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44229
![Western Blot: Papain Antibody [NBP2-44229]](https://resources.bio-techne.com/images/products/Papain-Antibody-Western-Blot-NBP2-44229-img0002.jpg "Western Blot: Papain Antibody [NBP2-44229]")
Key Product Details
Species Reactivity
Plant
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya. (Uniprot: P00784)
Reactivity Notes
Cross reactivity against Papain from other sources may occur but has not been specifically determined
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Papain Antibody - BSA Free
Western Blot: Papain Antibody [NBP2-44229]
Western Blot: Papain Antibody [NBP2-44229] - Analysis using the Biotin conjugate of NBP2-44229. Detection of Lane 1: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40![Western Blot: Papain Antibody [NBP2-44229]](https://resources.bio-techne.com/images/products/Papain-Antibody-Western-Blot-NBP2-44229-img0001.jpg "Western Blot: Papain Antibody [NBP2-44229]")
Western Blot: Papain Antibody [NBP2-44229]
Western Blot: Papain Antibody [NBP2-44229] - lane 1: Papain. Load: 50 ng per lane.Applications for Papain Antibody - BSA Free
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:500-1:3000
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Papain
Alternate Names
Allergen Car p 1, Papaya proteinase I, PPI
UniProt
Additional Papain Products
Product Documents for Papain Antibody - BSA Free
Product Specific Notices for Papain Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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