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p70 S6 Kinase beta/S6K2 Antibody

Catalog # NB100-1545 | Novus Biologicals a Bio-Techne Brand
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NB100-1545

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for p70 S6 Kinase beta/S6K2 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 430 and the C-terminus (residue 482) of human Ribosomal Protein S6 Kinase, 70kda, polypeptide 2 using the numbering given in entry NP_003943.2 (GeneID 6199).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p70 S6 Kinase beta/S6K2 Antibody

Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody NB100-1545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Whole cell lysate (5 and 15 ug) from HeLa cells. Antibody used at 0.04 ug/ml for WB.

Applications for p70 S6 Kinase beta/S6K2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 -6 ug/mg of lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using NB100-1545 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p70 S6 Kinase beta

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.

Alternate Names

KLS, p70-beta, RPS6KB2, STK14B

Entrez Gene IDs

6199 (Human)

Gene Symbol

RPS6KB2

UniProt

Product Documents for p70 S6 Kinase beta/S6K2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p70 S6 Kinase beta/S6K2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for p70 S6 Kinase beta/S6K2 Antibody

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  • Q: I wondered if your S6k2 antibody cross reacted with S6K1 or is it specific to S6K2 NB100-1545

    A: There is no mention of specifically crossreacting with any other protein but the epitope recognized by this antibody is found between aa430 and 482 of the human Ribosomal protein S6 kinase. Our lab said that there is some similarity between S6K1 and S6K2 in the sequences of the immunizing peptide. This antibody preferentially binds to S6K2 (as seen in data sheet) and it is possible under certain conditions that it would cross-react with S6K1.

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