p35 baculovirus Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56153

Novus Biologicals, part of Bio-Techne
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NB100-56153
NB100-56153SS
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Key Product Details

Species Reactivity

Validated:

Drosophila, Silkworm, Virus

Cited:

Insect - Drosophila, Moth - Bombyx mori (Silkworm), Virus

Applications

Validated:

Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all p35 baculovirus Antibodies »

Product Specifications

Immunogen

Full-length recombinant p35 baculovirus protein was used as immunogen.

Reactivity Notes

Multiple species cross reactivity. Silkworm reactivity reported in scientific literature (PMID: 24486953). Drosophila reactivity reported in scientific literature (PMID: 30413561). Not yet tested in other species.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p35 baculovirus Antibody - BSA Free

Western Blot: p35 baculovirus Antibody [NB100-56153]

Western Blot: p35 baculovirus Antibody [NB100-56153]

Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.

Applications for p35 baculovirus Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 21514385)

Immunohistochemistry-Frozen

Assay Dependent

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
p35 baculovirus may be observed as one or multiple bands on western blot: p35 baculovirus is cleaved during its reaction with caspases. This results in cleavage of p35; 10 and 25 kDa fragments have been described. Additional breakdown p35 products may also result.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p35 baculovirus

The baculoviruses are a large, diverse family of DNA viruses that have evolved a number of mechanisms to manipulate thier insect hosts. One of these is the ability to regulate apoptosis during infection by expressing proteins that can inhibit caspase activation, including the caspase inhibitor p35 and the inhibitor of apoptosis (IAP) proteins (reviewed in Clem, 2005; Clarke and Clem, 2003; and Iller, 1997). The p35 baculovirus protein strongly inhibits caspase enzymatic activity, and is the the most broadly acting caspase inhibitor protein known. p35 baculovirus forms essentially irreversible complexes with its target caspases in a process that is accompanied by the cleavage of p35, generating two fragments of approximately 10 kDa and 25 kDa. These cleavage fragments remain associated with caspases and thereby block caspase activity. The ability of p35 to inhibit caspases along with the central role of caspases in the apoptotic process enables p35 baculovirus to block apoptosis in a phylogentically broad range of cells, and in response to a wide variety of apoptotic induction signals. For example, over expression of p35 in mammalian, insect, and nematode cells results in resistance to apoptosis. This antibody recognizes p35 baculovirus; p35 baculovirus migrates at ~35 kDa on SDS-PAGE.

Alternate Names

p35 Baculovirus, p35-Baculovirus

Additional p35 baculovirus Products

Product Documents for p35 baculovirus Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p35 baculovirus Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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