p21/CIP1/CDKN1A Antibody (A8C11-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32692
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A8C11-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within full length of human p21/CIP1/CDKN1A. (Uniprot: P38936)
Localization
Nucleus, Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p21/CIP1/CDKN1A Antibody (A8C11-R)
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (A8C11-R) [NBP3-32692]
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (A8C11-R) [NBP3-32692] - Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Mouse anti-p21/CIP1/CDKN1A antibody (NBP3-32692) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32692) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for p21/CIP1/CDKN1A Antibody (A8C11-R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200-1:400
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p21/CIP1/CDKN1A
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
Additional p21/CIP1/CDKN1A Products
Product Documents for p21/CIP1/CDKN1A Antibody (A8C11-R)
Product Specific Notices for p21/CIP1/CDKN1A Antibody (A8C11-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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