Otx2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86533
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Otx2 Antibody - BSA Free (NBP1-86533) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-Otx2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Otx2 Antibody - BSA Free
![Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]](https://resources.bio-techne.com/images/products/Otx2-Antibody-Immunohistochemistry-Paraffin-NBP1-86533-img0004.jpg "Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]")
Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]
Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.![Otx2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Otx2 Antibody - BSA Free [NBP1-86533]](https://resources.bio-techne.com/images/products/nbp1-86533_rabbit-polyclonal-otx2-antibody-256202591397.jpg "Chromatin Immunoprecipitation-exo-Seq: Otx2 Antibody - BSA Free [NBP1-86533]")
Chromatin Immunoprecipitation-exo-Seq: Otx2 Antibody - BSA Free [NBP1-86533]
ChIP-Exo-Seq composite graph for Anti-OTX2 (NBP1-86533) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
Otx2 Antibody [NBP1-86533] -
Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.Applications for Otx2 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 25870833).
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Otx2
Long Name
Orthodenticle Homolog 2
Alternate Names
homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2
Gene Symbol
OTX2
Additional Otx2 Products
Product Documents for Otx2 Antibody - BSA Free
Product Specific Notices for Otx2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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