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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Opossum, Primate, Rabbit

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 14D5

Format

Azide and BSA Free

Concentration

2 mg/ml

Product Summary for NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

Immunogen

Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Modification

p Ser552

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

Western Blot: NHE3/SLC9A3 Antibody (14D5) - Azide and BSA Free [NBP2-80877] - Detection of NHE3 [p Ser552] in human kidney lysate. Image from the standard format of this antibody.
Immunohistochemistry: NHE3/SLC9A3 Antibody (14D5) - Azide and BSA Free [NBP2-80877] - Mouse intestine, image courtesy of verified customer review. Image from the standard format of this antibody.
Simple Western: NHE3/SLC9A3 Antibody (14D5) - Azide and BSA Free [NBP2-80877] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

Application
Recommended Usage

ELISA

: 1:100-1:2000

Flow Cytometry

reported in scientific literature (PMID 21255452)

Immunocytochemistry/ Immunofluorescence

: 1:50-1:1000

Immunohistochemistry

: 1:50-1:1000

Immunoprecipitation

1:10-1:500

Knockdown Validated

reported in scientific literature (PMID 31276916)

Knockout Validated

reported in scientific literature (PMID 31276916)

Simple Western

: 1:1000

Western Blot

: 1-5 ug/ml
Application Notes
In Western Blot, a band can be seen at approx. 85 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE3/SLC9A3

Na+/H+ exchanger (NHE) family members are integral membrane proteins which play a key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and the small intestine, followed by the kidneys and the stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Gene Symbol

SLC9A3

Product Documents for NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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