N4BP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37688
![Western Blot: N4BP1 Antibody [NBP2-37688]](https://resources.bio-techne.com/images/products/N4BP1-Antibody-Western-Blot-NBP2-37688-img0001.jpg "Western Blot: N4BP1 Antibody [NBP2-37688]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody maps to a region between residue 250 to 300 of human NEDD4 Binding Protein 1 using the numbering given in entry NP_694574.3 (GeneID 9683).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for N4BP1 Antibody - BSA Free
Western Blot: N4BP1 Antibody [NBP2-37688]
Western Blot: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: N4BP1 Antibody [NBP2-37688]](https://resources.bio-techne.com/images/products/N4BP1-Antibody-Immunoprecipitation-NBP2-37688-img0002.jpg "Immunoprecipitation: N4BP1 Antibody [NBP2-37688]")
Immunoprecipitation: N4BP1 Antibody [NBP2-37688]
Immunoprecipitation: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for N4BP1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: N4BP1
Alternate Names
FLJ31821, KIAA0615, MGC176730, NEDD4 binding protein 1, NEDD4-binding protein 1
Gene Symbol
N4BP1
UniProt
Additional N4BP1 Products
Product Documents for N4BP1 Antibody - BSA Free
Product Specific Notices for N4BP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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