MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14011
![Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011]](https://resources.bio-techne.com/images/products/MYCL1-L-Myc-Antibody-PCRP-MYCL-2D5-Azide-and-BSA-Free-Protein-Array-NBP3-14011-img0001.jpg "Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-MYCL-2D5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human MYCL1/L-Myc protein (Uniprot: P12524)
Localization
Nucleus, Nucleoplasm, Mitotic chromosome
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
40.33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13977).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free
Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011]
Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MYCL1/L-Myc Antibody (PCRP-MYCL-2D5).![MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] -](https://resources.bio-techne.com/images/products/nbp3-14011_mouse-mycl1-l-myc-mab-pcrp-mycl-2d5-azide-and-bsa-free-295202516354533.jpg "Immunocytochemistry/ Immunofluorescence: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] -")
Immunocytochemistry/ Immunofluorescence: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] -
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).![MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free Flow Cytometry: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] -](https://resources.bio-techne.com/images/products/nbp3-14011_mouse-mycl1-l-myc-mab-pcrp-mycl-2d5-azide-and-bsa-free-29520251637717.jpg "Flow Cytometry: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] -")
Flow Cytometry: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free [NBP3-14011] -
Flow cytometric analysis of PFA-fixed HeLa cells. MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).Applications for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MYCL1/L-Myc
MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5'-CACGTG-3'. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis.
Long Name
v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived
Alternate Names
L-Myc, LMyc
Gene Symbol
MYCL
Additional MYCL1/L-Myc Products
Product Documents for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free
Product Specific Notices for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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